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An Improved Method for DNA Transformation of Intact Cells of Bacillus subtilis by Electroporation
https://doi.org/10.57375/00000502
https://doi.org/10.57375/000005020ec03379-f2c8-4ba9-9342-eb0f76087f33
| 名前 / ファイル | ライセンス | アクション |
|---|---|---|
KJ00000201581.pdf (505.0 kB)
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| Item type | 紀要論文 / Departmental Bulletin Paper(1) | |||||
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| 公開日 | 2009-04-10 | |||||
| タイトル | ||||||
| タイトル | An Improved Method for DNA Transformation of Intact Cells of Bacillus subtilis by Electroporation | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
| 資源タイプ | departmental bulletin paper | |||||
| ID登録 | ||||||
| ID登録 | 10.57375/00000502 | |||||
| ID登録タイプ | JaLC | |||||
| 著者 |
KUSAOKE, Hideo
× KUSAOKE, Hideo× KADOWAKI, Yasuyuki |
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| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | An electroporation procedure for the plasmid-mediated genetic transformation of intact cells of Bacillus subtilis vas performed. The method was optimized for B. subtilis ISWl214,and the transformation of ISW1214 , with plasmid pUB110,using the mixture of 0.3 M sucrose I mM CaCl_2 and 1 DM sodium citrate as a buffer for pulsation, results in a maximum efficiency of 2.8 x 104 transfornants per μg DNA, with a single pulse duration of 500 μsec with an initial electric field strength of 7 kV/cm. Transformation of ISW1214 occurred at comparable frequencies with pUB110(4.5 kbp), pC194(2.9 kbp), and pE194(3.7 kbp) plasmids. Plasmid DNA isolated from transformants , , as indistinguishable from authentic preparation of plasmid DNA on gel electrophoretic analysis. The electroporation was simple, rapid, and sampled at 10 μ1-small volume of cell suspension(～IxlO^<10> cells/ml, 4 pg DNA/μl). | |||||
| 書誌情報 |
福井工業大学研究紀要. 第一部 号 22, p. 223-233, 発行日 1992-03-20 |
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| 出版者 | ||||||
| 出版者 | 福井工業大学 | |||||
| ISSN | ||||||
| 収録物識別子タイプ | ISSN | |||||
| 収録物識別子 | 2868571 | |||||
| 書誌レコードID | ||||||
| 識別子タイプ | NCID | |||||
| 関連識別子 | TF00008961 | |||||
| 著者版フラグ | ||||||
| 出版タイプ | VoR | |||||
| 出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||
