{"created":"2023-06-20T13:42:54.709348+00:00","id":508,"links":{},"metadata":{"_buckets":{"deposit":"aef45a17-da13-45ad-8675-6331f4760d99"},"_deposit":{"created_by":2,"id":"508","owners":[2],"pid":{"revision_id":0,"type":"depid","value":"508"},"status":"published"},"_oai":{"id":"oai:fut.repo.nii.ac.jp:00000508","sets":["1"]},"author_link":["10220","10221"],"item_10002_biblio_info_7":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"1992-03-20","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"22","bibliographicPageEnd":"233","bibliographicPageStart":"223","bibliographic_titles":[{"bibliographic_title":"福井工業大学研究紀要. 第一部"}]}]},"item_10002_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"An electroporation procedure for the plasmid-mediated genetic transformation of intact cells of Bacillus subtilis vas performed. The method was optimized for B. subtilis ISWl214,and the transformation of ISW1214 , with plasmid pUB110,using the mixture of 0.3 M sucrose I mM CaCl_2 and 1 DM sodium citrate as a buffer for pulsation, results in a maximum efficiency of 2.8 x 104 transfornants per μg DNA, with a single pulse duration of 500 μsec with an initial electric field strength of 7 kV/cm. Transformation of ISW1214 occurred at comparable frequencies with pUB110(4.5 kbp), pC194(2.9 kbp), and pE194(3.7 kbp) plasmids. Plasmid DNA isolated from transformants , , as indistinguishable from authentic preparation of plasmid DNA on gel electrophoretic analysis. The electroporation was simple, rapid, and sampled at 10 μ1-small volume of cell suspension(～IxlO^<10> cells/ml, 4 pg DNA/μl).","subitem_description_type":"Abstract"}]},"item_10002_identifier_registration":{"attribute_name":"ID登録","attribute_value_mlt":[{"subitem_identifier_reg_text":"10.57375/00000502","subitem_identifier_reg_type":"JaLC"}]},"item_10002_publisher_8":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"福井工業大学"}]},"item_10002_relation_11":{"attribute_name":"書誌レコードID","attribute_value_mlt":[{"subitem_relation_type_id":{"subitem_relation_type_id_text":"TF00008961","subitem_relation_type_select":"NCID"}}]},"item_10002_source_id_9":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"2868571","subitem_source_identifier_type":"ISSN"}]},"item_10002_version_type_20":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"KUSAOKE, Hideo"}],"nameIdentifiers":[{"nameIdentifier":"10220","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"KADOWAKI, Yasuyuki"}],"nameIdentifiers":[{"nameIdentifier":"10221","nameIdentifierScheme":"WEKO"}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2023-04-12"}],"displaytype":"detail","filename":"KJ00000201581.pdf","filesize":[{"value":"505.0 kB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"KJ00000201581.pdf","url":"https://fut.repo.nii.ac.jp/record/508/files/KJ00000201581.pdf"},"version_id":"b062d242-5f73-4f57-9e88-855d12e83167"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"departmental bulletin paper","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"An Improved Method for DNA Transformation of Intact Cells of Bacillus subtilis by Electroporation","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"An Improved Method for DNA Transformation of Intact Cells of Bacillus subtilis by Electroporation"}]},"item_type_id":"10002","owner":"2","path":["1"],"pubdate":{"attribute_name":"公開日","attribute_value":"2009-04-10"},"publish_date":"2009-04-10","publish_status":"0","recid":"508","relation_version_is_last":true,"title":["An Improved Method for DNA Transformation of Intact Cells of Bacillus subtilis by Electroporation"],"weko_creator_id":"2","weko_shared_id":-1},"updated":"2023-06-20T14:31:00.715865+00:00"}