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Gene Tranfer into Some Species of Bacillus by Electroporation
https://doi.org/10.57375/00000684
https://doi.org/10.57375/00000684f9be64fb-6bbf-4c0f-9801-627fee1a512f
| 名前 / ファイル | ライセンス | アクション |
|---|---|---|
KJ00000201772.pdf (356.7 kB)
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| Item type | 紀要論文 / Departmental Bulletin Paper(1) | |||||
|---|---|---|---|---|---|---|
| 公開日 | 2009-04-10 | |||||
| タイトル | ||||||
| タイトル | Gene Tranfer into Some Species of Bacillus by Electroporation | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
| 資源タイプ | departmental bulletin paper | |||||
| ID登録 | ||||||
| ID登録 | 10.57375/00000684 | |||||
| ID登録タイプ | JaLC | |||||
| 著者 |
OHSE, Morimasa
× OHSE, Morimasa× TAKATO, Syoei× KUSATAKE, Hideo |
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| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | Electroporation was applied to ten spesies of Bacillus. It was found that transformation of three strains of B. licheniformis, B. cereus, and B. subtilis with plasmid DNA (pUB110) was possible and resulted in maximum efficiencies of 1.0x 10^3 1.2 x 10^2,and 9.3 x 10^4 per μg DNA, respectively, under optimal pulse conditions of an electric field strength of 7 kV/cm and a duration of 500 μS with a single squared pulse at O 'C. The transformants were confirmed to harbor the same intact plasmid by agarose gel electrophoresis. | |||||
| 書誌情報 |
福井工業大学研究紀要. 第一部 号 26, p. 219-226, 発行日 1996-03-20 |
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| 出版者 | ||||||
| 出版者 | 福井工業大学 | |||||
| ISSN | ||||||
| 収録物識別子タイプ | ISSN | |||||
| 収録物識別子 | 2868571 | |||||
| 書誌レコードID | ||||||
| 識別子タイプ | NCID | |||||
| 関連識別子 | TF00009143 | |||||
| 著者版フラグ | ||||||
| 出版タイプ | VoR | |||||
| 出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||
