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oai:fut.repo.nii.ac.jp:00001932 2023-06-20T14:04:21Z 1

Immobilization of Invertase on Carboxymethylchitosan and Chitosan KUSAOKE, Hideo 15803 An immobilization method using carboxymethylchitosan prepared from chitosan as an insoluble carrier was investigated. Invertase was immobilized on carboxymethylchitosan using the bifunctional agent, glutaraldehyde. The most active preparation was based on the enzyme bound carboxymethylchitosan with low degree of substitution (d.s.) such as 0.41. Its activity yield was 24.1%. The activity of the immobilized enzyme decreased as the d.s. of carboxymethyl groups increased. Compared with free invertase, immobilized enzyme exhibited similar pH optimum but more activity at the range of pH 5-7. Immobilized enzyme improved thermal stability. The immobilized enzyme retained ca. 70% of the initial activity even after the repeated use of 10 times. departmental bulletin paper 福井工業大学 1988-06-15 VoR application/pdf 福井工業大学研究紀要 18 253 259 2868571 https://fut.repo.nii.ac.jp/record/1932/files/KJ00000147243.pdf https://doi.org/10.57375/00001926 https://fut.repo.nii.ac.jp/records/1932 eng TF00011985